MPD: Data set: Crusio1

Crusio1: Spatial learning, grooming, response to novelty, inter-male aggression, and hippocampal neuroanatomy in 53 BXD recombinant inbred strains of mice (2018)

References (chronological)

1. Delprato A, Bonheur B, Algéo MP, Rosay P, Lu L, Williams RW, Crusio WE. Systems genetic analysis of hippocampal neuroanatomy and spatial learning in mice. Genes Brain Behav. 2015 Nov;14(8):591-606. doi: 10.1111/gbb.12259. Epub 2015 Nov 8. PubMed 26449520

2. Delprato A, Algéo MP, Bonheur B, Bubier JA, Lu L, Williams RW, Chesler EJ, Crusio WE. QTL and systems genetics analysis of mouse grooming and behavioral responses to novelty in an open field. Genes Brain Behav. 2017 Nov;16(8):790-799. doi: 10.1111/gbb.12392. Epub 2017 Jun 22. PubMed 28544613

3. Delprato A, Bonheur B, Algéo MP, Murillo A, Dhawan E, Lu L, Williams RW, Crusio WE. A quantitative trait locus on chromosome 1 modulates intermale aggression in mice. Genes Brain Behav. 2018 Sep;17(7):e12469. doi: 10.1111/gbb.12469. Epub 2018 Apr 27. PubMed 29457871

Crusio1 downloads
• Download Crusio1 project data set animal data, as uploaded
• Download Crusio1 animal data matrix with factor-related expansion applied as necessary
• Download Crusio1 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Crusio1 measures:

MA:0000168 brain
MA:0000191 hippocampus
MP:0000771 abnormal brain size
MP:0000807 abnormal hippocampus morphology
MP:0001259 abnormal body weight
MP:0001362 abnormal anxiety-related response
MP:0001392 abnormal locomotor behavior
MP:0001440 abnormal grooming behavior
MP:0001463 abnormal spatial learning
MP:0002061 abnormal aggression-related behavior
MP:0013189 abnormal exploration in a new environment
MP:0020147 abnormal defecation amount
VT:0000807 hippocampus morphology trait
VT:0001259 body mass
VT:0001392 locomotor behavior trait
VT:0001440 grooming behavior trait
VT:0002176 brain mass
VT:0010462 defecation behavior trait
VT:0010471 exploratory behavior trait
VT:0010716 anxiety-related behavior trait
VT:0010726 wildness behavior trait
VT:0010727 spatial reference memory trait
VT:0015014 aggression-related behavior trait

Investigators	Anna Delprato University of Bordeaux, Pessac FRANCE Wim E Crusio University of Bordeaux, Pessac FRANCE
Participants	Bonheur B, Algéo MP, Rosay P, Lu L, Williams RW, Bubier JA, Chesler EJ, Murillo A, Dhawan E
Contact	Wim E Crusio wim_crusio@yahoo.com Lab web site
Acknowledgements	Funding provided by NIH MH072920, AA016662, AA013499, AA018776, AA021951; Neuro-BSIK Mouse Phenomics Consortium (BSIK03053)
Project type	Phenotype strain survey data set
MPD identifiers	Crusio1 MPD:620
Data changelog	No updates/corrections. Initial release date: 07/2018.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Procedures conducted:

• body weight
• open field test		Locomotor activity and exploratory behavior. 20 min test.
• radial maze		Spatial learning.
• behavior observation		Inter-male agression.
• organ weights		Brain weight.
• histopathology		Hippocampal histology.

Mice: BXD w/par 55 strains ♀♂ age 10-16wks 1 experimental group

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)

Crusio1:1	Crusio1	body weight	(none)	body weight [g] bw 62001	BXD w/par	55	both	10-13wks	N=8

Crusio1:3	Crusio1	open field test	(none)	percentage of total time spent in center, 20 min test [%] center_time 62003	BXD w/par	55	both	10-12wks	N=8

Crusio1:4	Crusio1	open field test	(none)	distance run in center, 20 min test [cm] center_dist 62004	BXD w/par	55	both	10-12wks	N=8

Crusio1:5	Crusio1	open field test	(none)	percentage of total time spent in thigmotaxis zone, 20 min test [%] periphery_time 62005	BXD w/par	55	both	10-12wks	N=8

Crusio1:6	Crusio1	open field test	(none)	distance run in thigmotaxis zone, 20 min test [cm] periphery_dist 62006	BXD w/par	55	both	10-12wks	N=8

Crusio1:7	Crusio1	open field test	(none)	percentage of total distance run in thigmotaxis zone, 20 min test [%] periphery_dist_pct 62007	BXD w/par	55	both	10-12wks	N=8

Crusio1:8	Crusio1	open field test	(none)	total locomotor activity, 20 min test [cm] activity 62008	BXD w/par	55	both	10-12wks	N=8

Crusio1:9	Crusio1	open field test	(none)	number of leanings against wall, 20 min test [n] lean 62009	BXD w/par	55	both	10-12wks	N=8

Crusio1:10	Crusio1	open field test	(none)	number of rearings, forepaws not resting against wall, 20 min test [n] rear 62010	BXD w/par	55	both	10-12wks	N=8

Crusio1:11	Crusio1	open field test	(none)	number of jumps (all 4 paws leave floor), 20 min test [n] jump 62011	BXD w/par	55	both	10-12wks	N=8

Crusio1:12	Crusio1	open field test	(none)	number of fecal boli deposited, 20 min test [n] defec 62012	BXD w/par	55	both	10-12wks	N=8

Crusio1:13	Crusio1	open field test	(none)	frequency of bouts of grooming, 20 min test [n] groom_freq 62013	BXD w/par	55	both	10-12wks	N=8

Crusio1:14	Crusio1	open field test	(none)	total duration of grooming, 20 min test [s] groom_dur 62014	BXD w/par	55	both	10-12wks	N=8

Crusio1:15	Crusio1	open field test	(none)	mean duration of grooming bouts, 20 min test [s] groom_bout 62015	BXD w/par	55	both	10-12wks	N=8

Crusio1:16	Crusio1	radial maze	(none)	time to complete task [s] task_time 62016 day 1 day 2 day 3 day 4 day 5	BXD w/par	55	both	10-13wks	N=8

Crusio1:21	Crusio1	radial maze	(none)	number of different arms visited during first 8 arm choices of a trial [n] num_arms 62021 day 1 day 2 day 3 day 4 day 5	BXD w/par	55	both	10-13wks	N=8

Crusio1:26	Crusio1	radial maze	(none)	number of different arms visited during first 8 arm choices of a trial minus 5.3 (number expected by chance) [n] num_arms_adj 62026 day 3 day 4 day 5 mean	BXD w/par	55	both	10-13wks	N=8

Crusio1:30	Crusio1	radial maze	(none)	number of errors made before all 8 rewards collected [n] errors 62030 day 1 day 2 day 3 day 4 day 5 sum of days 3 4 5	BXD w/par	55	both	10-13wks	N=8

Crusio1:36	Crusio1	radial maze	(none)	mean duration per arm visit (including errors) [s] visit_time 62036 day 3 day 4 day 5 mean	BXD w/par	55	both	10-13wks	N=8

Crusio1:40	Crusio1	behavior observation	(none)	attack latency [s] latency 62040 day 1 day 2	BXD w/par	55	m	12-16wks	N=8

Crusio1:42	Crusio1	behavior observation	(none)	whether an animal attacks (0=no, 1=yes) [score] attack 62042 day 1 day 2	BXD w/par	55	m	12-16wks	N=8

Crusio1:44	Crusio1	behavior observation	(none)	whether an animal attacks (0=not either day, 1=yes on one or both days) [score] attack_combine 62044	BXD w/par	54	m	12-16wks	N=8

Crusio1:45	Crusio1	organ weights	(none)	brain weight [g] brain_wt 62045	BXD w/par	55	both	12-16wks	N=8

Crusio1:46	Crusio1	organ weights	(none)	brain weight as percentage of total body weight [%] brain_wt_pct 62046	BXD w/par	55	both	12-16wks	N=8

Crusio1:48	Crusio1	histopathology	(none)	total hippocampus size (arbitrary units) hippocampus 62048 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:50	Crusio1	histopathology	(none)	size of intra- and infrapyramidal mossy fibers (arbitrary units) iipmf 62050 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:52	Crusio1	histopathology	(none)	percentage of hippocampus that is Intra- and infrapyramidal mossy fibers [%] iipmf_pct 62052 left right mean	BXD w/par	55	both	12-16wks	N=4

Crusio1:55	Crusio1	histopathology	(none)	size of hilus (arbitrary units) hilus 62055 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:57	Crusio1	histopathology	(none)	percentage of hippocampus that is hilus [%] hilus_pct 62057 left right mean	BXD w/par	55	both	12-16wks	N=4

Crusio1:60	Crusio1	histopathology	(none)	size of suprapyramidal mossy fibers (arbitrary units) supra 62060 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:62	Crusio1	histopathology	(none)	percentage of hippocampus that is suprapyramidal mossy fibers [%] supra_pct 62062 left right mean	BXD w/par	55	both	12-16wks	N=4

Crusio1:65	Crusio1	histopathology	(none)	size of stratum pyramidale (arbitrary units) pyr 62065 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:67	Crusio1	histopathology	(none)	percentage of hippocampus that is stratum pyramidale [%] pyr_pct 62067 left right mean	BXD w/par	55	both	12-16wks	N=4

Crusio1:70	Crusio1	histopathology	(none)	size of stratum oriens (arbitrary units) oriens 62070 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:72	Crusio1	histopathology	(none)	percentage of hippocampus that is stratum oriens [%] oriens_pct 62072 left right mean	BXD w/par	55	both	12-16wks	N=4

Crusio1:75	Crusio1	histopathology	(none)	size of stratum radiatum (arbitrary units) rad 62075 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:77	Crusio1	histopathology	(none)	percentage of hippocampus that is stratum radiatum [%] rad_pct 62077 left right mean	BXD w/par	55	both	12-16wks	N=4

Crusio1:80	Crusio1	histopathology	(none)	size of stratum lacunosum-moleculare (arbitrary units) lacun 62080 left right	BXD w/par	55	both	12-16wks	N=4

Crusio1:82	Crusio1	histopathology	(none)	percentage of hippocampus that is stratum lacunosum-moleculare [%] lacun_pct 62082 left right mean	BXD w/par	55	both	12-16wks	N=4