Source of Mice: DO mice consisted of approximately 850 male and female adult mice from eight DO generations (G4-G11). The study used five independent cohorts of 100-200 non-sibling mice, and each cohort having half the animals from the first litter in the respective generation, and half were from the second litter. Mice were weaned at 3 weeks of age.
Age (wks): 3-30 wks at testing
Test Facility: The Jackson Laboratory, Bar Harbor, ME, USA
Feed: Standard rodent chow or high fat high sucrose (HFHS) diet
Water: Acidified water ad libitum
Bedding: Pine bedding
Husbandry: Weaned, three week old animals are housed in same-sex, pressurized, individually ventilated cages (Thoren #11 Duplex II; Thoren Caging Systems, Hazelton, PA)
Cage Density: 5
Temperature (°C): Not specified
Humidity (%): Not specified
Light / Dark Cycle: 12:12h (lights on 0600h)
Care and Use Statement: All animal procedures were approved by the Animal Care and Use Committee at The Jackson Laboratory (Animal Use Summary # 06006)