Age (wks): 8-24 wks
Test Facility: The Jackson Laboratory G3B
Feed: Sterilized 6% fat 5K52 chow, ad libitum (Lab Diet/PMI Nutrition, St. Lousi MO)
Water: Acidified (pH2.5-3) with vitamin K, ad libitum
Bedding: Pine Shavings
Cage Type, Distributor, Material, Size: Plastic duplex pressurized individually vented Thoren cage #3 (30.80 x 30.80 x 15.5 cm). The ventilated cage providing a minimum of 60 air changes per hour with each cage.
Husbandry: Bedding and water changed weekly
Cage Density: 1-5
Cage Enrichment: Nestlet and shepard shack
Temperature (°C): 18-23
Humidity (%): 40-60%
Light / Dark Cycle: 12:12h
Health Status and Monitoring: Intermediate Barrier, sentines tested quarterly.
Care and Use Statement: All animal protocols were reviewed and approved by The Jackson Laboratory Institutional Anicmal Care Committee.