ITP survival analysis:   rapamycin

Intervention:   rapamycin     mTOR complex repressor, anti-aging
Trial code: Rapa_lo Dose: 4.7 ppm initiated at age: 9 mo Sex: both Cohort: C2009
Trial code: Rapa_mid Dose: 14 ppm initiated at age: 9 mo Sex: both Cohort: C2009
Trial code: Rapa_hi Dose: 42 ppm initiated at age: 9 mo Sex: both Cohort: C2009
This compound had a significant effect on survival:   4.7 ppm females p < 0.0001; 14 ppm females p < 0.0001; 14 ppm males p = 0.0034; 42 ppm both sexes p < 0.0001

Results are based on the use of pooled factor(s) that is/are stratified within the survival analysis model.

All test sites combined – female
Rapa_lo effect on survival, females, test site = All
Log-rank test chisq = 48.8394 p < 0.0001
Wang-Allison test odds ratio = 9.7884 p < 0.0001
Quantiles Rapa_lo Control % difference
25% died before day: 856 778 +10 %
Median lifespan (days) 1035 881 +17 %
75% died before day: 1140 988 +15 %
90% died before day: 1218 1066 +14 %
Max lifespan observed (days) 1322 1229
Number of mice at start 115 242
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, females, test site = All
Log-rank test chisq = 89.5564 p < 0.0001
Wang-Allison test odds ratio = 17.8911 p < 0.0001
Quantiles Rapa_mid Control % difference
25% died before day: 933 778 +20 %
Median lifespan (days) 1079 881 +22 %
75% died before day: 1202 988 +22 %
90% died before day: 1287 1066 +21 %
Max lifespan observed (days) 1412 1229
Number of mice at start 116 242
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, females, test site = All
Log-rank test chisq = 142.653 p < 0.0001
Wang-Allison test odds ratio = 17.8163 p < 0.0001
Quantiles Rapa_hi Control % difference
25% died before day: 1050 778 +35 %
Median lifespan (days) 1130 881 +28 %
75% died before day: 1214 988 +23 %
90% died before day: 1278 1066 +20 %
Max lifespan observed (days) 1323 1229
Number of mice at start 120 242
Animal censor events are denoted by + marks in the plot
All test sites combined – male
Rapa_lo effect on survival, males, test site = All
Log-rank test chisq = 1.6494 p = 0.199
Wang-Allison test odds ratio = 1.5593 p = 0.1805
Quantiles Rapa_lo Control % difference
25% died before day: 565 616 -8 %
Median lifespan (days) 834 807 +3 %
75% died before day: 1013 973 +4 %
90% died before day: 1160 1094 +6 %
Max lifespan observed (days) 1464 1338
Number of mice at start 150 295
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, males, test site = All
Log-rank test chisq = 8.5846 p = 0.0034
Wang-Allison test odds ratio = 2.3931 p = 0.0068
Quantiles Rapa_mid Control % difference
25% died before day: 624 616 +1 %
Median lifespan (days) 884 807 +10 %
75% died before day: 1054 973 +8 %
90% died before day: 1178 1094 +8 %
Max lifespan observed (days) 1298 1338
Number of mice at start 142 295
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, males, test site = All
Log-rank test chisq = 23.9886 p < 0.0001
Wang-Allison test odds ratio = 3.1087 p = 0.0009
Quantiles Rapa_hi Control % difference
25% died before day: 683 616 +11 %
Median lifespan (days) 984 807 +22 %
75% died before day: 1110 973 +14 %
90% died before day: 1184 1094 +8 %
Max lifespan observed (days) 1348 1338
Number of mice at start 120 295
Animal censor events are denoted by + marks in the plot


By individual test site:

TJL – female
TJL – female      
Rapa_lo effect on survival, females, test site = TJL
Log-rank test chisq = 21.0818 p < 0.0001
Wang-Allison test odds ratio = 22.6911 p = 0.0003
Quantiles Rapa_lo Control % difference
25% died before day: 897 768 +17 %
Median lifespan (days) 1050 894 +17 %
75% died before day: 1138 976 +17 %
90% died before day: 1219 1051 +16 %
Max lifespan observed (days) 1322 1197
Number of mice at start 28 60
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, females, test site = TJL
Log-rank test chisq = 16.2388 p < 0.0001
Wang-Allison test odds ratio = 22.6911 p = 0.0003
Quantiles Rapa_mid Control % difference
25% died before day: 901 768 +17 %
Median lifespan (days) 985 894 +10 %
75% died before day: 1183 976 +21 %
90% died before day: 1278 1051 +22 %
Max lifespan observed (days) 1412 1197
Number of mice at start 28 60
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, females, test site = TJL
Log-rank test chisq = 28.3864 p < 0.0001
Wang-Allison test odds ratio = 22.2954 p = 0.0002
Quantiles Rapa_hi Control % difference
25% died before day: 958 768 +25 %
Median lifespan (days) 1078 894 +21 %
75% died before day: 1193 976 +22 %
90% died before day: 1283 1051 +22 %
Max lifespan observed (days) 1291 1197
Number of mice at start 32 60
Animal censor events are denoted by + marks in the plot
TJL – male
TJL – male      
Rapa_lo effect on survival, males, test site = TJL
Log-rank test chisq = 0.4194 p = 0.5172
Wang-Allison test odds ratio = 1.2481 p = 0.7779
Quantiles Rapa_lo Control % difference
25% died before day: 540 606 -11 %
Median lifespan (days) 761 807 -6 %
75% died before day: 943 941 0 %
90% died before day: 1106 1061 +4 %
Max lifespan observed (days) 1217 1338
Number of mice at start 54 101
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, males, test site = TJL
Log-rank test chisq = 0.2635 p = 0.6077
Wang-Allison test odds ratio = 1.5851 p = 0.4109
Quantiles Rapa_mid Control % difference
25% died before day: 536 606 -12 %
Median lifespan (days) 718 807 -11 %
75% died before day: 1023 941 +9 %
90% died before day: 1156 1061 +9 %
Max lifespan observed (days) 1271 1338
Number of mice at start 52 101
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, males, test site = TJL
Log-rank test chisq = 6.8204 p = 0.009
Wang-Allison test odds ratio = 3.7119 p = 0.0191
Quantiles Rapa_hi Control % difference
25% died before day: 634 606 +5 %
Median lifespan (days) 835 807 +3 %
75% died before day: 1142 941 +21 %
90% died before day: 1272 1061 +20 %
Max lifespan observed (days) 1344 1338
Number of mice at start 47 101
Animal censor events are denoted by + marks in the plot
UM – female
UM – female      
Rapa_lo effect on survival, females, test site = UM
Log-rank test chisq = 18.0495 p < 0.0001
Wang-Allison test odds ratio = 9.7698 p = 0.0002
Quantiles Rapa_lo Control % difference
25% died before day: 856 797 +7 %
Median lifespan (days) 1065 886 +20 %
75% died before day: 1160 1008 +15 %
90% died before day: 1217 1088 +12 %
Max lifespan observed (days) 1287 1229
Number of mice at start 43 90
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, females, test site = UM
Log-rank test chisq = 34.5487 p < 0.0001
Wang-Allison test odds ratio = 36.3041 p < 0.0001
Quantiles Rapa_mid Control % difference
25% died before day: 940 797 +18 %
Median lifespan (days) 1090 886 +23 %
75% died before day: 1222 1008 +21 %
90% died before day: 1285 1088 +18 %
Max lifespan observed (days) 1389 1229
Number of mice at start 44 90
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, females, test site = UM
Log-rank test chisq = 55.5326 p < 0.0001
Wang-Allison test odds ratio = 9.4804 p = 0.0003
Quantiles Rapa_hi Control % difference
25% died before day: 1072 797 +35 %
Median lifespan (days) 1160 886 +31 %
75% died before day: 1198 1008 +19 %
90% died before day: 1274 1088 +17 %
Max lifespan observed (days) 1323 1229
Number of mice at start 44 90
Animal censor events are denoted by + marks in the plot
UM – male
UM – male      
Rapa_lo effect on survival, males, test site = UM
Log-rank test chisq = 5.1703 p = 0.023
Wang-Allison test odds ratio = 2.4966 p = 0.1454
Quantiles Rapa_lo Control % difference
25% died before day: 822 742 +11 %
Median lifespan (days) 990 925 +7 %
75% died before day: 1146 1077 +6 %
90% died before day: 1298 1151 +13 %
Max lifespan observed (days) 1464 1325
Number of mice at start 48 95
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, males, test site = UM
Log-rank test chisq = 1.1518 p = 0.2832
Wang-Allison test odds ratio = 1.8041 p = 0.3606
Quantiles Rapa_mid Control % difference
25% died before day: 826 742 +11 %
Median lifespan (days) 1004 925 +9 %
75% died before day: 1100 1077 +2 %
90% died before day: 1203 1151 +5 %
Max lifespan observed (days) 1269 1325
Number of mice at start 42 95
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, males, test site = UM
Log-rank test chisq = 8.6236 p = 0.0033
Wang-Allison test odds ratio = 1.8033 p = 0.3344
Quantiles Rapa_hi Control % difference
25% died before day: 954 742 +29 %
Median lifespan (days) 1075 925 +16 %
75% died before day: 1142 1077 +6 %
90% died before day: 1270 1151 +10 %
Max lifespan observed (days) 1348 1325
Number of mice at start 36 95
Animal censor events are denoted by + marks in the plot
UT – female
UT – female      
Rapa_lo effect on survival, females, test site = UT
Log-rank test chisq = 12.0323 p = 0.0005
Wang-Allison test odds ratio = 6.3687 p = 0.0019
Quantiles Rapa_lo Control % difference
25% died before day: 850 750 +13 %
Median lifespan (days) 950 866 +10 %
75% died before day: 1112 994 +12 %
90% died before day: 1218 1062 +15 %
Max lifespan observed (days) 1287 1213
Number of mice at start 44 92
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, females, test site = UT
Log-rank test chisq = 39.4028 p < 0.0001
Wang-Allison test odds ratio = 9.6976 p = 0.0002
Quantiles Rapa_mid Control % difference
25% died before day: 1010 750 +35 %
Median lifespan (days) 1095 866 +26 %
75% died before day: 1196 994 +20 %
90% died before day: 1314 1062 +24 %
Max lifespan observed (days) 1379 1213
Number of mice at start 44 92
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, females, test site = UT
Log-rank test chisq = 59.5087 p < 0.0001
Wang-Allison test odds ratio = 37.1192 p < 0.0001
Quantiles Rapa_hi Control % difference
25% died before day: 1072 750 +43 %
Median lifespan (days) 1137 866 +31 %
75% died before day: 1225 994 +23 %
90% died before day: 1259 1062 +19 %
Max lifespan observed (days) 1321 1213
Number of mice at start 44 92
Animal censor events are denoted by + marks in the plot
UT – male
UT – male      
Rapa_lo effect on survival, males, test site = UT
Log-rank test chisq = 0.3569 p = 0.5503
Wang-Allison test odds ratio = 1.1759 p = 0.7708
Quantiles Rapa_lo Control % difference
25% died before day: 466 497 -6 %
Median lifespan (days) 665 704 -6 %
75% died before day: 926 903 +3 %
90% died before day: 1025 1013 +1 %
Max lifespan observed (days) 1261 1133
Number of mice at start 48 99
Animal censor events are denoted by + marks in the plot
Rapa_mid effect on survival, males, test site = UT
Log-rank test chisq = 12.2962 p = 0.0005
Wang-Allison test odds ratio = 4.8862 p = 0.0066
Quantiles Rapa_mid Control % difference
25% died before day: 600 497 +21 %
Median lifespan (days) 854 704 +21 %
75% died before day: 1038 903 +15 %
90% died before day: 1178 1013 +16 %
Max lifespan observed (days) 1298 1133
Number of mice at start 48 99
Animal censor events are denoted by + marks in the plot
Rapa_hi effect on survival, males, test site = UT
Log-rank test chisq = 8.679 p = 0.0032
Wang-Allison test odds ratio = 4.2206 p = 0.0216
Quantiles Rapa_hi Control % difference
25% died before day: 650 497 +31 %
Median lifespan (days) 893 704 +27 %
75% died before day: 1046 903 +16 %
90% died before day: 1098 1013 +8 %
Max lifespan observed (days) 1126 1133
Number of mice at start 37 99
Animal censor events are denoted by + marks in the plot


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Statistical definitions